Document 1840
 DOCN  M94A1840
 TI    A new supplemental assay for the characterization and simultaneous
       detection of HIV antibody and antigen.
 DT    9412
 AU    Delagneau JF; Basse JM; Morat L; Prigent V; Sanjuan A; SANOFI
       Diagnostics PASTEUR, Marnes la Coquette, France.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):44 (abstract no. 148B). Unique
       Identifier : AIDSLINE ICA10/94370735
 AB    OBJECTIVE: To provide a highly sensitive assay by simultaneous detection
       of anti ENV HIV-1 and/or HIV-2 antibody and/or GAG antigen with
       characterization of each reactive analyte. METHODS: Well documented
       serial bleeds of HIV-1 seroconverters were tested for p24 antigen, gp41
       and HIV2 gp36 antibodies with a unitary membrane test strip containing
       specific bands for each analyte to be detected. This assay, called
       DECISCAN HIV Ab/Ag, is based on a sandwich ELISA format using HIV1 and
       HIV2 ENV antigens (synthetic peptides and recombinant glycoprotein) for
       antibody detection and monoclonal antibodies for p24 detection. RESULTS:
       In all seroconverters tested, HIV-1 antibody detection bands were
       reactive in the same bleeds as in 3rd generation antibody assays. In
       three patients, p24 was detected in at least one bleed prior to antibody
       detection. Three other seroconversions became antibody and antigen
       positive in the same bleed. One seroconverters became antibody reactive
       without antigen detection. No reactivity was observed in 200 HIV
       antibody negative samples from normal blood donors. DISCUSSION AND
       CONCLUSION: The new DECISCAN HIV Ab/Ag supplemental assay allows an
       early diagnosis of HIV seroconverters based on highly sensitive anti ENV
       HIV antibody detection improved by simultaneous p24 antigen detection.
       This assay gives useful additonal data for patients with low antibody
       reactivity or WB indeterminate results.
 DE    Enzyme-Linked Immunosorbent Assay/*METHODS/STATISTICS & NUMER  DATA
       Gene Products, env/BLOOD/IMMUNOLOGY  Gene Products, gag/BLOOD/IMMUNOLOGY
       Human  HIV Antibodies/*BLOOD  HIV Antigens/*BLOOD/IMMUNOLOGY  HIV Core
       Protein p24/BLOOD/IMMUNOLOGY  HIV Envelope Protein gp41/BLOOD/IMMUNOLOGY
       HIV Seropositivity/DIAGNOSIS/IMMUNOLOGY/MICROBIOLOGY
       HIV-1/IMMUNOLOGY/ISOLATION & PURIF  HIV-2/IMMUNOLOGY/ISOLATION & PURIF
       Sensitivity and Specificity  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).